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How to Prepare Vero Cells for Storage & Use

Vero cells come from the kidney epithetical cells that originated from the African Green Monkey. Making them an animal cell line.

The first Vero cells were extracted from the African Green Monkey by Y. Yasumura and Y. Kawakita in 1962. By the end of the 60s Vero cells where being used all over the world specifically in virology labs.

Vero cell lines are still extensively used for screening for viruses, bacterial toxins, and for parasitic studies. Vero cells maintain their characteristics of normal cells, particularly the cell contact inhibition. When the Vero cells reach convergence in the cells mono layer, the cells must be subcultured, or they start to die off.

Lab Made Vero Cells

Once the cells are harvested, they must be subcultured a day or two before being frozen. This ensures that the cells are in the log metrical phase of growth when frozen to make sure when they are thawed, they will grow and not need to be discarded. The cells are frozen at -80°C or in liquid nitrogen.

Preparing the Vero Cells for Storage

When using a mammalian cell line tenacious cultures must be passaged during the log phase. Otherwise, they will be useless when unfrozen. Human cells stop growing when they merge. Transmuted cells can continue growing after they have reached confluence. They generally start to deteriorate shortly after two replications. Cells in deferment must be passaged during their log-phase growth. Once the cells reach confluency, any cells that are suspended they clump together and appear cloudy when they are swirled in the culture container.

When performing cell passaging, observing a stringent schedule guarantees reproducible cell behavior. Achieving the steady rate of growth yielding suitable for cell form from a provided seeding concentration.

Divergences from the development patterns established generally suggest that the culture is damaged which can be caused by deterioration, a poorly functioning of your culture system is not functioning properly which could also be due to a temperature that is not optimal, or a cultural medium that is too old. It is recommended that a detailed culture log is kept, detailing the subculture, and feeding schedules, dissociation followed, media used, seeding, morphological observations, split ratios, and any antibiotic uses.

Isolating Passaged Cells

The cells need to be detached from the culture’s vial. This process is done mechanically or enzymatic. First, gently shake the container or use a pipetting technique. Usually, a warm bath of 37degree temperature is used to thaw the cells.

The cells can only be passaged up to fifteen times. Then must be discarded. Some cells may not be any good at all if they were contaminated, or not frozen at the correct time. Vero cells are very important especially when creating vaccinations.

Sources

Thermofisher

ResearchGate

Oxford

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